ABSTRACT
Advanced mRNA vaccines play vital roles against SARS-CoV-2. However, most current mRNA delivery platforms need to be stored at -20 °C or -70 °C due to their poor stability, which severely restricts their availability. Herein, we develop a lyophilization technique to prepare SARS-CoV-2 mRNA-lipid nanoparticle vaccines with long-term thermostability. The physiochemical properties and bioactivities of lyophilized vaccines showed no change at 25 °C over 6 months, and the lyophilized SARS-CoV-2 mRNA vaccines could elicit potent humoral and cellular immunity whether in mice, rabbits, or rhesus macaques. Furthermore, in the human trial, administration of lyophilized Omicron mRNA vaccine as a booster shot also engendered strong immunity without severe adverse events, where the titers of neutralizing antibodies against Omicron BA.1/BA.2/BA.4 were increased by at least 253-fold after a booster shot following two doses of the commercial inactivated vaccine, CoronaVac. This lyophilization platform overcomes the instability of mRNA vaccines without affecting their bioactivity and significantly improves their accessibility, particularly in remote regions.
ABSTRACT
This study explored the user-generated translation activity in the context of the Chinese online social media. It focused on Bilibili content creators dedicated to translating public comments on China-related videos posted on international social media platforms such as YouTube, and creating videos featuring Chinese translated comments. Viewing their translation actions as events in a collective activity system, the authors collected data from 30 participants through a questionnaire and follow-up interviews with two participants who have recently worked on videos about China?s fight against Covid-19. All the data were analysed by using Engeström?s activity theory model to create an activity model showing how their user-generated translation activity was conducted. In this study, we observed that the participants, as the non-professional translation community on the Chinese online social media, were breaking down linguistic borders for fans and viewers, and postulated the possible interaction between the user-generated translation work and their better understanding of how the world saw China through grassroots expression of opinions.